Pharmaceutical compositions containing a 1-(cyano-phenoxy)-2-hydroxy-3-alkylamino-propane and method of use

ABSTRACT

Pharmaceutical compositions containing as an active ingredient a compound of the formula   wherein R is branched alkyl of 5 to 8 carbon atoms containing a quaternary carbon atom which is attached directly or through an alkylene chain of 1 to 4 carbon atoms to the amino nitrogen atom, and R2 is hydrogen or chlorine, OR A NON-TOXIC, PHARMACOLOGICALLY ACCEPTABLE ACID ADDITION SALT THEREOF; AND A METHOD OF USING THE SAME AS Beta -ADRENOLYTICS AND HYPOTENSIVES.

United States Patent [1 Koppe et al.

[ Oct. 21, 1975 PHARMACEUTICAL COMPOSITIONS CONTAINING A l-(CYANO-PHENOXY)-2-HYDROXY-3- ALKYLAMINO-PROPANE AND METHOD OF USE [75] Inventors: Herbert Kiippe; Werner Kummer;

Helmut Stiihle; Karl Zeile, all of lngelheim am Rhein; Albrecht Engelhardt, Main (Rhine), all of Germany [73] Assignee: Boehringer Ingelheim GmbH,

' Ingelheim am Rhein, Germany 22 Filed: Jan. 16, 1975 [21] Appl. No.: 541,430

Related U.S.' Application Data [60] Division of SCI. No. 204,316, Dec. 2, 1971, Pat. No. 3,872,147, which is a continuation of Ser. No.

781,985, Dec. 6, 1968, abandoned.

{30] Foreign Application Priority Data 3,740,444 6/1973 Koppe et al. 424/304 Primary Examiner-Sam Rosen Attorney, Agent, or Firm-Hammond & Littell [57] ABSTRACT Pharmaceutical compositions containing as an active ingredient a compound of the formula wherein R is branched alkyl of 5 to 8 carbon atoms A containing a quaternary carbon atom which is attached directly or through an alkylene chain of 1 to 4 carbonatoms to the amino nitrogen atom, and

R is hydrogen or chlorine,

or a non-toxic, pharmacologically acceptable acid addition salt thereof, and a method of using the same as B-adrenolytics and hypotensives.

.6 Claims, N0 Drawings PHARMACEUTICAL COMPOSITIONS CONTAINING A l-(CYANO-PHENOXY)-2-HYDROXY-3- ALKYLAMINO-PROPANE AND METHOD OF USE This is a division of copending application Ser. No. 204,316, filed Dec. 2, 1971, now US. Pat. No. 3,872,147, which in turn is a continuation of application Ser. No. 781,985, filed Dec. 6, l968, now aban- 1 doned.

This invention relates to novel pharmaceutical compositions containing as an active ingredient a l-(cyanophenoxy)-2-hydroxy-3alkylamino-propane or a nontoxic acid addition salt thereof, as well as to a method of using the same as B-adrenolytics and hypotensives.

More particularly, the present invention relates to novel pharmaceutical dosage unit compositions containing as an active ingredient a compound of the formula Method A By reacting a compound of the formula wherein R, has the same meanings as in formula I and Z is or CH(OI-I)-CI-I I-Ial, where Hal is halogen, with an alkylamine -of the formula NH, --R (III) wherein R has the same meanings as in formula I, in a manner and under conditions which are customary for such reactions.

Method B By splitting off an easily removable protective group from a compound of the formula wherein R and R have the same meanings as in formula I and G is a hydrolytically removable protective 0 group, such as acyl or acetal.

Method C By converting the substituent A in a compound of the formula into a cyano substituent. R and R in formula V have the same meanings as in formula I, and A may have any one of the following meanings:

CONI-I or CI-I=NOH, which are convertible into cyano by dehydration; or Amino, which is convertible into cyano by diazotization and heating with copper(I)-cyanide. The conversion of a compund of the formula V into a compound of the formula I is effected by applying the required known reaction, i.e., dehydration or diazotization and subsequent heating with a copper(l)cyanide, to the particular compound of the formula V.

Method D By introducing a chlorine substituent onto the phenyl ring of a compound of the formula CN OH wherein R has the same meaning as in formula I.

The introduction of the chlorine substituent may be effected by reacting a compound of the formula VI with a mixture of concentrated hydrogen peroxide and hydrochloric acid at elevated temperatures.

The starting compounds required for methods A through D are either known compounds or may be prepared according to known procedures.

For instance, an epoxide of the formula II may be prepared by reacting epichlorohydrin with a corresponding phenol or phenolate of the formula R,- (VII) wherein R has the same meanings as in formula I and X is hydrogen or a cation, especialy an alkali metal cation. An epoxide of the formula II, in turn, may be used for the preparation of other starting compounds; for example, a halohydrin of the formula II may be prepared by reacting the corresponding epoxide with a hydrohalic acid.

A compound of the formula IV may be prepared by reacting a halohydrin of the formula II with a com- 5 pound which forms a protective group G, such as vinyl ether or dihydropyran, and subsequently reacting the compound of the formula formed thereby, wherein R and G have the same meanings as in formula IV and Hal is halogen, with an amine of the formula III.

Finally, compounds of the formulas V and VI may be prepared pursuant to method A described herein, i.e., starting from a corresponding phenol by way of the corresponding l-(cyano-phenoxy)-2,3-epoxypropane and reaction of the latter with an alkylamine of the formula III.

The compounds according to the present invention comprise an asymmetric carbon atom and therefore occur as racemic mixtures as well as in the form of optically active antipodes. The latter may be obtained by separating the racemic mixture with an aid of customary auxiliary acids, such as dibenzoyl-D-tartaric acid or D'-3-bromocamphor-8-sulfonic acid, or also by using the corresponding optically active starting compound.

The compounds of the formula I are organic bases and therefore form acid addition salts with inorganic or organic acids. Examples of non-toxic, pharmacologically acceptable acid addition salts are those formed with hydrochloric acid, hydrobromic acid, sulfuric acid, methane-sulfonic acid, maleic acid, acetic acid, oxalic acid, lactic acid, tartaric acid, 8-chlorotheophylline and the like. Such acid addition salts may be obtained by conventional methods, for instance, by dissolving the free base in a suitable inert solvent'and acidifying the solution with the desired inorganic or organic 45 acid.

The following examples illustrate the preparation of compounds of the formula I.

EXAMPLE 1 l-( o-cyano-phenoxy)-2-hydroxy-3-[ a,a-dimethyl-npropyl)-amino]-propane and its hydrochloride by Method A 14 gm of 82% a,a-dimethyl-n-propyl-amine were added to a solution of 10.5 gm (0.06 mol) of l-(o- 55 cyanophenoxy)-2,3-epoxy-propane in 80 cc of ethanol, the mixture was allowed to stand for 24 hours at C, and thereafter it was refluxed on a boiling water bath for about 3 hours. Subsequently, the ethanol was distilled off in vacuo, the residue was digested with dilute hydrochloric acid, the insoluble matter was separated, and the acid solution was made alkaline with sodium hydroxide. The base precipitated thereby was taken up in ether, the organic phase was dried over magnesium sulfate, and the ether was distilled off. The residue, l- 65 (o-cyano-phenoxy)-2-hydroxy 3-[(a,a-dimethyl-npropyl)-amino]-propane, was dissolved in acetonitrile, the solution was acidified with ethereal hydrochloric acid, and the crystalline precipitate was recrystallized from ethanol/ether, yielding 7.6 gm of the colorless crystalline hydrochloride, m.p. l34l 36C, of the formula CN a Using a procedure analogous to that described in Example 1, the following additional l-(o-cyano-phenoxy)-2-hydroxy-3-alkylamino-propanes,and their hydrochlorides of the formula i were prepared from l-(o-cyano-phenoxy)-2,3-epoxypropane and the corresponding primary amine of the formula III above:

Ex. R M.P. (Hydrochloride) No. I C

Cl l:J3CH-, I63

in, CH, 4 i-C.H, I48 150 11, CH, CH, 7 JZCH, -CH, 145 1411 H H, a --C(Cz H9, 157 15x 9 (lI-iC,H, 177

10 cH,-iC,H, 137 139 ":1 CH, 11 l-tert.C.H. 2l8 220 -continued Ex.

M.P. (Hydrochloride) No. "C

Using a procedure analogous to that described in Example 1, the following additional 1-('cyano-phenoxy)- 2-hydroxy-3-alkyl-amino-propanes and their hydrochlorides of the formula were prepared from the corresponding l-(cyanophenoxy)-2,3-propane and primary amine of the formula III:

l-( o-cyano-phenoxy)-2-hydroxy-3-[(a,a-dimethyl-npentyl)-amino]-propane and its hydrochloride by method A 2.1 gm (0.087 mol) of l-(o-cyano-phenoxy)-2- hydroxy-3-bromo-propane were dissolved in 50 cc of ethanol, 2 gm (0.0175 mol) of tert.heptylamine (a,adimethyl-n-pentyl-amine) were added to the solution, andthe mixture was refluxed for 2 hours. Thereafter, the ethanol was distilled off, the residue was digested with dilute NaOH, the aqueous alkaline mixture was extracted with ether, the ethereal extract solution was washed with water and dried over magnesiuim sulfate, and the ether was distilled off. 3 gm of raw l -(o-cyanophenoxy)-2-hydroxy-3-[(a,a-dimethyl-n-pentyl)- amino]-propane remained behind, which were dissolved in a small amount of ethanol, the resulting solution was acidified with etheral hydrochloric acid, and the precipitate formed thereby was recrystallized from ethanol/ether, yielding 2.8 gm of the hydrochloride, m.p. l44l45C, of the formula CN CH3 EXAMPLE 17 l-(o-cyano-p-chloro-phenoxy)-2-hydroxy-3-[(a-methyl-a-ethyl-n-butyl)-amino]-propane and its hydrochloride by method D 6.52 gm (0.02 mol) of 1-(o-cyano-phenoxy)-3-[(amethyl-a-ethyl-n-butyl)-amino]-propane hydrochloride were dissolved in 55 cc of concentrated hydrochloric acid, and then 2.7 gm (0.024 mol) of hydrogen peroxide were added dropwise to the solution at about 45C, whereby the internal temperature rose to 65C. The reaction mixture was thereafter stirred at 60C for 30 minutes}; then concentrated by evaporation in vacuo, madeipalkaline with dilute sodium hydroxide, and the oily product precipitated thereby was taken up 5 in ether. The ethereal solution was washed with water,

dried over magnesium sulfate, and evaporated to dryness in vacuol The solid residue was dissolved in ethyl acetate and recrystallized therefrom by addition of petroleum etherl The pure crystalline base, l-(o-cyano-pchloro-phenoxy)-2-hydroxy-3-[(a-methyl-a-ethyl-nbutyl)-amino]-propane, was dissolved in'ether, and the resulting solution was acidified with ethereal hydrochloric acid. The precipitate formed thereby was collected and recrystallized from acetonitrile/ether, yielding 2 gm of the-hydrochloride, m.p. 143145C, of the formula EXAMPLE l8 1-(m-cyano-phenoxy)-2-hydroxy-3-[ (a,a-dimethyl-nbutyl)-amino]-propane and its hydrochloride by method C 3.39 gm (0:01 'mol) of 1-(m-amino-phenoxy)-2- hydroxy-3-[ (,mq-dimethyl-n-butyl )-amino]-propane hydrochloride were dissolved in 3.5 cc of concentrated hydrochloric acid, and the solution was diluted with 20 cc of watenThe aqueous solution was cooled to 10C, and then, while stirring, a solution of 1.4 gm (0.02 mol) of NaNO; in 10 cc of water was added dropwise over a period of 15 minutes. Thereafter, the mixture was stirred for 30 minutes more at 10C, and then a solution of 5 gm of CuSO SHQO and 5.6 gm of potassium cyanide in 30 cc of water, heated to 90C, was added dropwise to the mixture at 90C over a period of 20 minutes, accompanied by stirring. Subsequently, the aqueous phase was decanted from resinous components which had formed and was then extracted with chloroform. The organic phase was washed with water, dried over magnesium sulfate and evaporated in vacuo. The residue, raw 1-(m-cyano-phenoxy)-2-hydroxy-3- [(a,a-dimethyl-n-butyl)-amino]-propane, was dissolved in a small amount of ethanol, the solution was acidified with ethereal hydrochloric acid, and the crystalline precipitate formed thereby was recrystallized from ethanol/ether, yielding 0.7 gm of the hydrochloride, m.p. 138l40C, of the formula The compounds embraced by formula I above and their non-toxic, pharmacologically acceptable acid addition salts, have useful pharmacodynamic properties. More particularly, they exhibit B-adrenolytic and hypotensive activities in warm-blooded animals, as confirmed by in vivo tests on guinea pigs. Thus, the compounds are useful for the treatment and prophylaxis of diseases of the coronary heart vessels and cardiac arrythmia, especially tachicardia, in warm-blooded animals.

For pharmaceutical purposes the compounds of the formula I or their non-toxic acid addition salts are administered to warm-blooded animals perorally or parenterally as sole active ingredients or in combination with other pharmacodynamically active ingredients, such as coronary dilators or sympathicomimetics, in customary dosage unit compositions, that is, compositions in dosage unit form consisting of essentiallyof an inert pharmaceutical carrier and one effective dosage unit of the active ingredient, such as tablets, coated pills, capsules, wafers, powders, solutions, suspensions, emulsions, syrups, suppositories and the like. One effective dosage unit of the compounds is from 0.0166 to 5.0 mgm/kg body weight, preferably 0.083 to 1.67 mgm/kg (peroral) or 0.0166 to 0.34 mgm/kg (parenteral).

The following examples illustrate a few dosage unit compositions comprising a compound of the formula I or a nontoxic acid addition salt thereof as an active ingredient and represent the best mode contemplated of putting the invention to practical use. The parts are parts by weight unless otherwise specified.

EXAMPLE 19 Tablets The tablet composition was compounded from the following ingredients:

l-(o-Cyano-phenoxy)-2-hydroxy-3-[(a.adimethyl-n-propyl)-amino]-propane hydrochloride 40.0 parts Corn starch 164.0 Secondary calcium phosphate 240.0 Magnesium stearate l.0

Total 445.0 parts EXAMPLE 2O Gelatin capsules The capsule filler composition was compounded from the following ingredients:

l-(o-Cyano-phenoxy)-2-hydroxy-3-[(01.11- dimethyl-n-butyl)-amino]-propane hydrochloride 25 .0 parts Corn starch .0

Total 200.0 parts Compounding procedure:'

The ingredients were intimately admixed with each other, and 200 mgmportions of the mixture were filled into gelatin capsules of suitable size. Each capsule contained 25 mgm of the phenoxy-amino-propanol compound and, when administered perorally to a warmblooded animal of about 60 kg body weight in need of such treatment, produced very good B-adrenolytic effects.

EXAMPLE 2] Hypodermic solution The solution was compounded from the following ingredients:

l-( m-Cyano-phenoxy)-2-hydroxy-3-[ (11,0:- clirnethyl-n-propyl )-amino]-propane hydrochloride 25 parts Sodium salt of EDTA 2 Distilled water q.s.ad 1000 by vol.

. EXAMPLE 22 'Sustained release pills The pill core composition was compounded from the following ingredients:

dimethyl-n-propyl)-amino]-propane hydrochloride 25.0 parts Carboxymethyl cellulose (CMC) 295.0

Stearic acid r i 20.0 Cellulose acetate phthalate (CAP) 40.0

' Total 380.0 parts Compoundingprocedure:

The propanol compound, the CMC and the stearic acid were intimately admixed with each other, the mixture was granulated in customary fashion with a solution of the CAP in 200 ccof a mixture ofethanol and ethyl acetate, and thegranulate was pressed into 380 mgm-pill cores which were then coated with a sugarcontaining 5% solution of polyvinyl-pyrrolidone in water. Each pill contained 25 mgm of the phenoxy-aminopropanol compound and, when administered perorally to a warm-blooded animal of about 60 kg body weight in need of such treatment, produced very good B- adrenolytic effects.

EXAMPLE 23 Tablets with combination of active ingredients The tablet composition was compounded from the following ingredients:

I-( o-Cyano-phenoxy )-2-hydroxy-3-[ 01,0:- dimethyl-n-propyl )-amino -propane hydrochloride 35 .0

2.6-Bis-(diethanolamino)-4,8-dipiperldino-pyrimido[5,4-dlpyrimidine 75.0 Lactose 164.0 Corn Starch 194.0 Colloidal silicic acid 14.0 Polyvinylpyrrolidone 6.0 Magnesium stearate 2.0 Soluble starch 10.0

Total 500.0 parts Compounding procedure:

The propanol compound, the pyrimidopyrimide compound, the lactose, the corn starch, the colloidal silicic acid and the polyvinylpyrrolidone were intimately admixed with each other, the mixture was granulated in customary fashion with an aqueous solution of the soluble starch, the granulate was admixed with the magnesium stearate, and the composition was pressed into 500 mgm-tablets. Each tablet contaned 35 mgm of the phenoxy-amino-propanol compound and 75 mgm of the pyrimido-pyrmidine compound and, when administered perorally to a warm-blooded animal of about 60 kg body weight in need of such treatment, produced very good B-adrenolytic and coronary dilating effects.

Analogous results were obtained when an equal amount of any one of the other compounds embraced by formula 1 above or a non-toxic acid addition salt thereof was substituted for the particular phenoxyamino-propanol compounds in Examples 19 through 23. Likewise, the amount of active ingredient in these examples may be varied to achieve the dosage unit range set forth above, and the amounts and nature of the inert pharmaceutical carrier ingredients may be varied to meet particular requirements.

While the present invention has been illustrated with the aid of certain specific embodiments thereof, it will be readily apparent to others skilled in the art that the invention is not limited to these particular embodiments, and that various changes and modifications may be made without departing from the spirit of the invention or the scope of the appended claims.

We claim:

1. A pharmaceutical dosage unit composition consisting essentially of an inert pharmaceutical carrier 10 and an effective B-adrenolytic or hypotensive amount of compound of the formula wherein R is branched alkyl of 5 to 8 carbon atoms containing a quaternary carbon atom which is attached directly or through an alkylene chain of l to 4 carbon atoms to the amino nitrogen atom, and

R is hydrogen or chlorione, or a non-toxic pharmacologically acceptable acid addition salt thereof.

2. A composition of claim 1, wherein said compound is l-(o-cyano-phenoxy)-2-hydroxy-3-[(a,a-dimethyl-npropyl)-amino]-propane or a non-toxic, pharmacologically acceptable acid addition salt thereof.

3. A composition of claim 1, wherein said compound is l-(o-cyano-phenoxy)-2-hydroxy-3-[(a,a-dimethyl-nbutyl)-amino]-propane or a non-toxic, pharmacologically acceptable acid addition salt thereof.

4. The method of blocking the B-adrenergic receptors and lowering the blood pressure in a warmblooded animal in need of such treatment, which comprises administering to said animal an effective B- adrenolytic or hypotensive amount of a compound of the formula I wherein R is branched alkyl of 5 to 8 carbon atoms containing a quaternary carbon atom which is attached directly or through an alkylene chain of l to 4 carbon atoms to the amino nitrogen atom, and R is hydrogen or chlorine, or a non-toxic, pharmacologically acceptable acid addition salt thereof.

5. The method of claim 4, wherein said compound is l-( o-cyano-phenoxy )-2-hydroxy-3-[ a,a-dimethyl-npropyl)-amino]-propane or a non-toxic, pharmacologically acceptable acid addition salt thereof.

6. The method of claim 4, wherein said compound is l-(o-cyanophenoxy)-2-hydroxy-3-[(a,a-dimethyl-nbutyl)-amino]-propane or a non-toxic, pharmacologically acceptable acid addition salt thereof. 

1. A PHARMACEUTICAL DOSAGE UNIT COMPOSITION CONSISTING ESSENTIALLY OF AN INERT PHARMACEUTICAL CARRIER AND AN EFFECTIVE B-ADRENOLYTIC OR HYPOTENSIVE AMOUNT OF COMPOUND OF THE FORMULA
 2. A composition of claim 1, wherein said compound is 1-(o-cyano-phenoxy)-2-hydroxy-3-(( Alpha , Alpha -dimethyl-n-propyl)-amino)-propane or a non-toxic, pharmacologically acceptable acid addition salt thereof.
 3. A composition of claim 1, wherein said compound is 1-(o-cyano-phenoxy)-2-hydroxy-3-(( Alpha , Alpha -dimethyl-n-butyl)-amino)-propane or a non-toxic, pharmacologically acceptable acid addition salt thereof.
 4. The method of blocking the Beta -adrenergic receptors and lowering the blood pressure in a warm-blooded animal in need of such treatment, which comprises administering to said animal an effective Beta -adrenolytic or hypotensive amount of a compound of the formula
 5. The method of claim 4, wherein said compound is 1-(o-cyano-phenoxy)-2-hydroxy-3-(( Alpha , Alpha -dimethyl-n-propyl)-amino)-propane or a non-toxic, pharmacologically acceptable acid addition salt thereof.
 6. The method of claim 4, wherein said compound is 1-(o-cyano-phenoxy)-2-hydroxy-3-(( Alpha , Alpha -dimethyl-n-butyl)-amino)-propane or a non-toxic, pharmacologically acceptable acid addition salt thereof. 